The active fractions from size-exclusion were loaded into a Mono S column or Sulfopropyl sepharose fast flow (SPFF) for further purification

The active fractions from size-exclusion were loaded into a Mono S column or Sulfopropyl sepharose fast flow (SPFF) for further purification. higher affinities to DNA substrates. In a dimeric enzyme, the two active sites work in tandem, with the faster site functioning before the slower one, and in the monomeric Continue Reading

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These antibodies have been shown to be able to recognize CD1d loaded with -GalCer and its analogs, while they do not recognize glycosyl ceramides having a -linked sugars moiety (iGb3, -GalCer (24:1), -psychosine, -GluCer, -glucopsychosine) or non-glycosyl ceramides (GalA-GAL, BbGL-2c, Glc-DAG-s2) that can be identified by the TCR of iNKT cells (26, 28, 29)

These antibodies have been shown to be able to recognize CD1d loaded with -GalCer and its analogs, while they do not recognize glycosyl ceramides having a -linked sugars moiety (iGb3, -GalCer (24:1), -psychosine, -GluCer, -glucopsychosine) or non-glycosyl ceramides (GalA-GAL, BbGL-2c, Glc-DAG-s2) that can be identified by the TCR of iNKT Continue Reading

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