The active fractions from size-exclusion were loaded into a Mono S column or Sulfopropyl sepharose fast flow (SPFF) for further purification. higher affinities to DNA substrates. In a dimeric enzyme, the two active sites work in tandem, with the faster site functioning before the slower one, and in the monomeric Continue Reading
MBT
These antibodies have been shown to be able to recognize CD1d loaded with -GalCer and its analogs, while they do not recognize glycosyl ceramides having a -linked sugars moiety (iGb3, -GalCer (24:1), -psychosine, -GluCer, -glucopsychosine) or non-glycosyl ceramides (GalA-GAL, BbGL-2c, Glc-DAG-s2) that can be identified by the TCR of iNKT cells (26, 28, 29)
These antibodies have been shown to be able to recognize CD1d loaded with -GalCer and its analogs, while they do not recognize glycosyl ceramides having a -linked sugars moiety (iGb3, -GalCer (24:1), -psychosine, -GluCer, -glucopsychosine) or non-glycosyl ceramides (GalA-GAL, BbGL-2c, Glc-DAG-s2) that can be identified by the TCR of iNKT Continue Reading