The binding of the precise antibodies was visualized with the addition of 100 l/well of tetramethylbenzidine (Dako TMB+ substrate-chromogen; Dakocytomation) substrate

The binding of the precise antibodies was visualized with the addition of 100 l/well of tetramethylbenzidine (Dako TMB+ substrate-chromogen; Dakocytomation) substrate. 96.7% and of 94.9%, respectively. Hence, data on the entire performance from the dental fluid IgM catch ELISA are in close contract with those of the serum IgM assay, Continue Reading

(H, I) The mice were sacrificed, and subcutaneous tumors were excised and weighed at the 28th day after inoculation

(H, I) The mice were sacrificed, and subcutaneous tumors were excised and weighed at the 28th day after inoculation. RNA was detected by qRT/PCR described above. 2.6. Vectors construction The cDNA encoding LINC00662 was PCR\amplified with Thermo Scientific Phusion Flash High\Fidelity PCR Master Mix (Thermo Fisher, Waltham, MA, USA) and Continue Reading