Staining of nuclei was performed using DAPI for 5 min and coverslips were mounted on slides utilizing a Prolong Anti-fade package (Invitrogen, P36930). Protein-protein interactions were visualized using the Duolink proximity ligation assay [73] program (Olink Bioscience) following manufacturers guidelines using anti-HBZ, anti-EZH2, anti SUZ12, anti-His, or anti-c-Myc antibodies. TM4SF18 Continue Reading