Mitochondrial Membrane Potential To further concur that C2 and C1 induced apoptosis, mitochondrial membrane potential or mitochondrial destabilization was measured in MCF-7 cells following treatment with C2 and C1. Cyto-Tox96 X assay (Anatech, Promega G 400) was utilized to judge the cytotoxic activity of C1 and C2 on A549 cells. Continue Reading