Recombinant GST-fusion proteins were portrayed in and purified by glutathione-Sepharose 4B chromatography. cleavage of GST-p47 with thrombin and purified by passing through glutathione-Sepharose 4B and benzamidine-Sepharose columns. Binding Assays The mammalian appearance plasmids pEBG (Tanaka (2000) was utilized. For the recognition of FP2, cells had been set in 4% paraformaldehyde Continue Reading