The binding of the precise antibodies was visualized with the addition of 100 l/well of tetramethylbenzidine (Dako TMB+ substrate-chromogen; Dakocytomation) substrate. 96.7% and of 94.9%, respectively. Hence, data on the entire performance from the dental fluid IgM catch ELISA are in close contract with those of the serum IgM assay, Continue Reading