Truncation mutants that end at the indicated amino acid number (untruncated wild-type DBT ends at amino acid 440) were cloned into the pMT vector with a MYC epitope at the C terminus and expressed in Drosophila S2 cells, with varying amounts of plasmid and induction by CuSO4

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Truncation mutants that end at the indicated amino acid number (untruncated wild-type DBT ends at amino acid 440) were cloned into the pMT vector with a MYC epitope at the C terminus and expressed in Drosophila S2 cells, with varying amounts of plasmid and induction by CuSO4. the mutations shorten Continue Reading