The culture-expanded AFS cells at 3 104/mL were induced in NH OsteoDiff Medium (Miltenyi Biotec) for 3 weeks

The culture-expanded AFS cells at 3 104/mL were induced in NH OsteoDiff Medium (Miltenyi Biotec) for 3 weeks. media-2 (EGM-2), the stem cells exhibited an endothelial-like morphology, formed cord-like structures when plated on Matrigel, and uptook acetylated LDL/lectin. Additionally, mRNA and protein levels of CD31 and von Willebrand factor (vWF) Continue Reading