The reaction was developed by the addition of TMB (3,3,5,5-tetramethyl-benzidine, Sigma) and stopped after 10?min incubation with 4?N H2SO4. block the binding of NoV VLPs to saliva, but not to D-Caco-2 cells. Blocking HBGAs on the surface of D-Caco-2 cells with specific monoclonal antibodies did not affect NoV VLP binding Continue Reading