Approximately 3 106 solitary cells were added per AggreWell?800 well in Essential 8? medium supplemented with the ROCK inhibitor Y-27632 (10 M, Selleckchem, S1049), centrifuged at 100 x g for 3 minutes to capture the cells in the microwells and incubated at 37oC with 5% CO2

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Approximately 3 106 solitary cells were added per AggreWell?800 well in Essential 8? medium supplemented with the ROCK inhibitor Y-27632 (10 M, Selleckchem, S1049), centrifuged at 100 x g for 3 minutes to capture the cells in the microwells and incubated at 37oC with 5% CO2. After 24 hours, spheroids Continue Reading