After checking the IPC analysis, the reaction combination was purified by a TFF system using a Sartocon Slice 200 ECO Hydrosart membrane (30 kDa; Sartorius) and conjugation buffer while DF buffer at an antibody concentration of 6.8 mg/mL to afford linker reoxidation product 4 (1.78 g, 100% yield) in conjugation Continue Reading